Description
RP1-Serpin-A13 is a polyclonal antibody made to the serine proteinase inhibitor Serpin-A13. The antibody is made to a synthetic peptide based on the aminoterminal end of mature human serpin-A13. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.
Use
Serpin-A13 is an A-clade serpin, the archetype of which is alpha-1 antitrypsin. The mode of action of most A-clade serpins involves a dramatic structural change after cleavage of a 'bait' region. The normal conformation of A-clade serpins is in a stressed state, and cleavage converts the molecule to a relaxed, more energetically favorable state. The structural change traps the proteinase that cleaves the reactive center loop (RCL), like a mousetrap. Other serpins of the A-clade are well known as circulating inhibitors of serine proteinases, principally in the coagulation cascade, but little is known to date about serpin-A13 specificity, localization or function. The sequence for serpin-A13 encodes a protein that terminates after helices 7-8, before the reactive center loop, thus serpin-A13 is not considered an active serine proteinase inhibitor. The abbreviated carboxyterminus has led some to postulate that serpin-A13 is a pseudogene, and that the protein is not expressed. Full length serpin-A12 sequence codes for a 307 amino acid protein, with a predicted mass of 34.9 kDa and a pI of 5.9. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY; NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 12 months at -20C.
Description
RP2-Serpin-A13 is a polyclonal antibody made to the serine proteinase inhibitor Serpin-A13. The antibody is made to a synthetic peptide based on helix 4 to helix 5 of human serpin-A13. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.
Use
Serpin-A13 is an A-clade serpin, the archetype of which is alpha-1 antitrypsin. The mode of action of most A-clade serpins involves a dramatic structural change after cleavage of a 'bait' region. The normal conformation of A-clade serpins is in a stressed state, and cleavage converts the molecule to a relaxed, more energetically favorable state. The structural change traps the proteinase that cleaves the reactive center loop (RCL), like a mousetrap. Other serpins of the A-clade are well known as circulating inhibitors of serine proteinases, principally in the coagulation cascade, but little is known to date about serpin-A13 specificity, localization or function. The sequence for serpin-A13 encodes a protein that terminates after helices 7-8, before the reactive center loop, thus serpin-A13 is not considered an active serine proteinase inhibitor. The abbreviated carboxyterminus has led some to postulate that serpin-A13 is a pseudogene, and that the protein is not expressed. Full length serpin-A12 sequence codes for a 307 amino acid protein, with a predicted mass of 34.9 kDa and a pI of 5.9. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY; NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 12 months at -20C.
Description
RP3-Serpin-A13 is a polyclonal antibody made to the serine proteinase inhibitor Serpin-A13. The antibody is made to a synthetic peptide based on helix 7 to helix 8 of human serpin-A13. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.
Use
Serpin-A13 is an A-clade serpin, the archetype of which is alpha-1 antitrypsin. The mode of action of most A-clade serpins involves a dramatic structural change after cleavage of a 'bait' region. The normal conformation of A-clade serpins is in a stressed state, and cleavage converts the molecule to a relaxed, more energetically favorable state. The structural change traps the proteinase that cleaves the reactive center loop (RCL), like a mousetrap. Other serpins of the A-clade are well known as circulating inhibitors of serine proteinases, principally in the coagulation cascade, but little is known to date about serpin-A13 specificity, localization or function. The sequence for serpin-A13 encodes a protein that terminates after helices 7-8, before the reactive center loop, thus serpin-A13 is not considered an active serine proteinase inhibitor. The abbreviated carboxyterminus has led some to postulate that serpin-A13 is a pseudogene, and that the protein is not expressed. Full length serpin-A12 sequence codes for a 307 amino acid protein, with a predicted mass of 34.9 kDa and a pI of 5.9. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY; NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 12 months at -20C.
