Description
RP1-Testisin is a polyclonal antibody made to the Serine proteinase testisin. The antibody is made to a synthetic peptide based on the propeptide domain of human testisin. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
Use
Testisin was first cloned from human eosinophils, and named eosinophil serine protease (ESP-1). The enzyme was found to be expressed at low levels in many tissues and cell lines, but the highest concentrations were found in the testis, and the name testisin applied. Testisin is not specific to testis, and the levels of testisin are found to drop in many testicular cancers due to hypermethylation, so testisin is thought to be a negative tumor regulator protein in testicular germ cell tumors. In ovarian cancer, however, testisin is reported to be highly expressed, and is thought to promote malignant transformation. Over expression of testisin in normal epithelial cells promotes tumor formation in soft gel assays and in animal tumor models. In sperm, testisin is thought to be localized to the apical surface of the sperm, and to act like acrosin in promoting sperm-egg fusion and fertilization. Testisin is a serine protease of the trypsin-like SA clan, in the S1A family of MEROPS classification, similar to other serine proteases such as hepsin, prosatsin, plasmin, and other S1A family members in containing the HDS catalytic triad in the catalytic domain, and with the catalytic serine held in the GDSGG motif. Testisin most closely resembles gamma tryptase and marapsin in overall sequence identity. Another group of serine proteases named the testis-specific serine proteases (TSP-1, TSP-2, TPS-5) are more distantly related to testisin, with 34-37% identity at the amino acid level. Considered a membrane associated protease, testisin is held in place not by a transmembrane domain as in the transmembrane serine proteinases, but by a GPI anchor in the carboxyterminal region. Currently there are 5 splice variants published which encode proteins of 300, 306, 312, 314 and 320 amino acids, and predicted mass of 33.5-35.5 kDa. Testisin is thought to be glycosylated in several positions, which adds 1-3 kDa to the apparent mass. Little is known about the relative expression or abundance of the different splice variants, but all are predicted to contain a competent catalytic triad, and are thought to be functional enzymes. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 6 months at 40C. For longer storage, store at -200C
Description
RP2-Testisin is a polyclonal antibody made to the Serine proteinase testisin. The antibody is made to a synthetic peptide based on the aminoterminal end of the catalytic domain of human testisin. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
Use
Testisin was first cloned from human eosinophils, and named eosinophil serine protease (ESP-1). The enzyme was found to be expressed at low levels in many tissues and cell lines, but the highest concentrations were found in the testis, and the name testisin applied. Testisin is not specific to testis, and the levels of testisin are found to drop in many testicular cancers due to hypermethylation, so testisin is thought to be a negative tumor regulator protein in testicular germ cell tumors. In ovarian cancer, however, testisin is reported to be highly expressed, and is thought to promote malignant transformation. Over expression of testisin in normal epithelial cells promotes tumor formation in soft gel assays and in animal tumor models. In sperm, testisin is thought to be localized to the apical surface of the sperm, and to act like acrosin in promoting sperm-egg fusion and fertilization. Testisin is a serine protease of the trypsin-like SA clan, in the S1A family of MEROPS classification, similar to other serine proteases such as hepsin, prosatsin, plasmin, and other S1A family members in containing the HDS catalytic triad in the catalytic domain, and with the catalytic serine held in the GDSGG motif. Testisin most closely resembles gamma tryptase and marapsin in overall sequence identity. Another group of serine proteases named the testis-specific serine proteases (TSP-1, TSP-2, TPS-5) are more distantly related to testisin, with 34-37% identity at the amino acid level. Considered a membrane associated protease, testisin is held in place not by a transmembrane domain as in the transmembrane serine proteinases, but by a GPI anchor in the carboxyterminal region. Currently there are 5 splice variants published which encode proteins of 300, 306, 312, 314 and 320 amino acids, and predicted mass of 33.5-35.5 kDa. Testisin is thought to be glycosylated in several positions, which adds 1-3 kDa to the apparent mass. Little is known about the relative expression or abundance of the different splice variants, but all are predicted to contain a competent catalytic triad, and are thought to be functional enzymes. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 6 months at 40C. For longer storage, store at -200C
Description
RP3-Testisin is a polyclonal antibody made to the Serine proteinase testisin. The antibody is made to a synthetic peptide based on the catalytic domain of human testisin, after the catalytic histidine residue. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
Use
Testisin was first cloned from human eosinophils, and named eosinophil serine protease (ESP-1). The enzyme was found to be expressed at low levels in many tissues and cell lines, but the highest concentrations were found in the testis, and the name testisin applied. Testisin is not specific to testis, and the levels of testisin are found to drop in many testicular cancers due to hypermethylation, so testisin is thought to be a negative tumor regulator protein in testicular germ cell tumors. In ovarian cancer, however, testisin is reported to be highly expressed, and is thought to promote malignant transformation. Over expression of testisin in normal epithelial cells promotes tumor formation in soft gel assays and in animal tumor models. In sperm, testisin is thought to be localized to the apical surface of the sperm, and to act like acrosin in promoting sperm-egg fusion and fertilization. Testisin is a serine protease of the trypsin-like SA clan, in the S1A family of MEROPS classification, similar to other serine proteases such as hepsin, prosatsin, plasmin, and other S1A family members in containing the HDS catalytic triad in the catalytic domain, and with the catalytic serine held in the GDSGG motif. Testisin most closely resembles gamma tryptase and marapsin in overall sequence identity. Another group of serine proteases named the testis-specific serine proteases (TSP-1, TSP-2, TPS-5) are more distantly related to testisin, with 34-37% identity at the amino acid level. Considered a membrane associated protease, testisin is held in place not by a transmembrane domain as in the transmembrane serine proteinases, but by a GPI anchor in the carboxyterminal region. Currently there are 5 splice variants published which encode proteins of 300, 306, 312, 314 and 320 amino acids, and predicted mass of 33.5-35.5 kDa. Testisin is thought to be glycosylated in several positions, which adds 1-3 kDa to the apparent mass. Little is known about the relative expression or abundance of the different splice variants, but all are predicted to contain a competent catalytic triad, and are thought to be functional enzymes. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 6 months at 40C. For longer storage, store at -200C
Description
RP4-Testisin is a polyclonal antibody made to the Serine proteinase testisin. The antibody is made to a synthetic peptide based on the catalytic domain of human testisin, after the catalytic aspartic acid residue. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
Use
Testisin was first cloned from human eosinophils, and named eosinophil serine protease (ESP-1). The enzyme was found to be expressed at low levels in many tissues and cell lines, but the highest concentrations were found in the testis, and the name testisin applied. Testisin is not specific to testis, and the levels of testisin are found to drop in many testicular cancers due to hypermethylation, so testisin is thought to be a negative tumor regulator protein in testicular germ cell tumors. In ovarian cancer, however, testisin is reported to be highly expressed, and is thought to promote malignant transformation. Over expression of testisin in normal epithelial cells promotes tumor formation in soft gel assays and in animal tumor models. In sperm, testisin is thought to be localized to the apical surface of the sperm, and to act like acrosin in promoting sperm-egg fusion and fertilization. Testisin is a serine protease of the trypsin-like SA clan, in the S1A family of MEROPS classification, similar to other serine proteases such as hepsin, prosatsin, plasmin, and other S1A family members in containing the HDS catalytic triad in the catalytic domain, and with the catalytic serine held in the GDSGG motif. Testisin most closely resembles gamma tryptase and marapsin in overall sequence identity. Another group of serine proteases named the testis-specific serine proteases (TSP-1, TSP-2, TPS-5) are more distantly related to testisin, with 34-37% identity at the amino acid level. Considered a membrane associated protease, testisin is held in place not by a transmembrane domain as in the transmembrane serine proteinases, but by a GPI anchor in the carboxyterminal region. Currently there are 5 splice variants published which encode proteins of 300, 306, 312, 314 and 320 amino acids, and predicted mass of 33.5-35.5 kDa. Testisin is thought to be glycosylated in several positions, which adds 1-3 kDa to the apparent mass. Little is known about the relative expression or abundance of the different splice variants, but all are predicted to contain a competent catalytic triad, and are thought to be functional enzymes. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 6 months at 40C. For longer storage, store at -200C
Description
RP5-Testisin is a polyclonal antibody made to the Serine proteinase testisin. The antibody is made to a synthetic peptide based on the carboxyterminal end of human testisin, after the catalytic aspartic acid residue. The antibody has been peptide-affinity purified, concentrated to 1 mg/ml, with the addition of 0.05% Sodium Azide as preservative, and 50% glycerol as cryoprotectant.
Use
Testisin was first cloned from human eosinophils, and named eosinophil serine protease (ESP-1). The enzyme was found to be expressed at low levels in many tissues and cell lines, but the highest concentrations were found in the testis, and the name testisin applied. Testisin is not specific to testis, and the levels of testisin are found to drop in many testicular cancers due to hypermethylation, so testisin is thought to be a negative tumor regulator protein in testicular germ cell tumors. In ovarian cancer, however, testisin is reported to be highly expressed, and is thought to promote malignant transformation. Over expression of testisin in normal epithelial cells promotes tumor formation in soft gel assays and in animal tumor models. In sperm, testisin is thought to be localized to the apical surface of the sperm, and to act like acrosin in promoting sperm-egg fusion and fertilization. Testisin is a serine protease of the trypsin-like SA clan, in the S1A family of MEROPS classification, similar to other serine proteases such as hepsin, prosatsin, plasmin, and other S1A family members in containing the HDS catalytic triad in the catalytic domain, and with the catalytic serine held in the GDSGG motif. Testisin most closely resembles gamma tryptase and marapsin in overall sequence identity. Another group of serine proteases named the testis-specific serine proteases (TSP-1, TSP-2, TPS-5) are more distantly related to testisin, with 34-37% identity at the amino acid level. Considered a membrane associated protease, testisin is held in place not by a transmembrane domain as in the transmembrane serine proteinases, but by a GPI anchor in the carboxyterminal region. Currently there are 5 splice variants published which encode proteins of 300, 306, 312, 314 and 320 amino acids, and predicted mass of 33.5-35.5 kDa. Testisin is thought to be glycosylated in several positions, which adds 1-3 kDa to the apparent mass. Little is known about the relative expression or abundance of the different splice variants, but all are predicted to contain a competent catalytic triad, and are thought to be functional enzymes. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 6 months at 40C. For longer storage, store at -200C
