Description
RP1-TLL-1 is a polyclonal antibody made to Tolloid-Like-1 (mTLL1). The antibody is made to a synthetic peptide based on the propeptide domain of the human sequence. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.
Use
TLL-1, also known as Tolloid-Like-1 is an extracellular zinc endopeptidase of the astacin family. The astacin family includes BMP-1, Astacin, Meprin-A and B, Tolloid-like proteins and Choriolysin. All contain the canonical HExxHxxxxxH zinc vinding motif in the catalytic metalloprotease domain. TLL-1 and TLL-2 are members of the BMP-1 subfamily, and the name BMP-1 reflects the involvement in the collagen deposition of growing bone (a collagen-rich environment). Procollagen C-peptidase cleaves the carboxy end from procollagen, to allow the formation of mature, triplehelical collagen. The aminoterminal end must also be cleaved by the Procollagen N-Proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP-1 are thought to lead to aberrant collagen processing and connective tissue disorders. TLL-1 also cleaves procollagen and chordin , although it appears that TLL-2 does not. Efficacy of processing of the different collagen forms by BMP-1, TLL-1 and TLL-2 is still being investigated. Pro-Lysyl oxidase, required for collagen cross linking, is also processed by the TLL enzymes. The BMP-1 family members are important both for collagen processing and for their role in processing proteins important in development, in particular the TGF-beta proteins. Substrate differences for the different enzymes are being elucidated, but it seems likely that the many different forms of these proteinases allow specificity in cleavage for the diverse range of connective and signaling molecules. A splice variant of BMP-1 referred to as mTolloid, the mammalian form of the Tolloid gene, and several isoforms forms of BMP-1 have been reported, with varying truncation at the carboxyterminal end. Tolloid-Like-1 and Tolloid-Like-2 share the same overall domain structure as BMP-1: a signal sequence, propeptide domain, then a catalytic metalloproteinase domain, two CUB domains followed by an EGF-like calcium binding domain, another CUB domain, another EGF-like domain followed by two CUB domains. The propeptide domains differ amongst the different enzymes, but the MP domain through the CUB3 domain is well conserved. The sequences begin to diverge at the second EGF-like domain, and the carboxyterminal end is where most of the diversity is amongst the different enzymes and slice variants. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. FOR RESEARCH USE ONLY; NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 6 months at 4C. For longer storage, store at -20C.
Description
RP2-TLL-1 is a polyclonal antibody made to Tolloid-Like-1 (mTLL1). The antibody is made to a synthetic peptide based on the third CUB domain of the human sequence. The antibody has been peptide-affinity purified, concentrated to 1.0 mg/ml, with the addition of 0.05% sodium azide as preservative and 50% glycerol as cryoprotectant.
Use
TLL-1, also known as Tolloid-Like-1 is an extracellular zinc endopeptidase of the astacin family. The astacin family includes BMP-1, Astacin, Meprin-A and B, Tolloid-like proteins and Choriolysin. All contain the canonical HExxHxxxxxH zinc vinding motif in the catalytic metalloprotease domain. TLL-1 and TLL-2 are members of the BMP-1 subfamily, and the name BMP-1 reflects the involvement in the collagen deposition of growing bone (a collagen-rich environment). Procollagen C-peptidase cleaves the carboxy end from procollagen, to allow the formation of mature, triplehelical collagen. The aminoterminal end must also be cleaved by the Procollagen N-Proteinase (PNP or ADAM-TS2). Defects in PNP have been linked to the skin disorder dermatosparaxis, and defects in BMP-1 are thought to lead to aberrant collagen processing and connective tissue disorders. TLL-1 also cleaves procollagen and chordin , although it appears that TLL-2 does not. Efficacy of processing of the different collagen forms by BMP-1, TLL-1 and TLL-2 is still being investigated. Pro-Lysyl oxidase, required for collagen cross linking, is also processed by the TLL enzymes. The BMP-1 family members are important both for collagen processing and for their role in processing proteins important in development, in particular the TGF-beta proteins. Substrate differences for the different enzymes are being elucidated, but it seems likely that the many different forms of these proteinases allow specificity in cleavage for the diverse range of connective and signaling molecules. A splice variant of BMP-1 referred to as mTolloid, the mammalian form of the Tolloid gene, and several isoforms forms of BMP-1 have been reported, with varying truncation at the carboxyterminal end. Tolloid-Like-1 and Tolloid-Like-2 share the same overall domain structure as BMP-1: a signal sequence, propeptide domain, then a catalytic metalloproteinase domain, two CUB domains followed by an EGF-like calcium binding domain, another CUB domain, another EGF-like domain followed by two CUB domains. The propeptide domains differ amongst the different enzymes, but the MP domain through the CUB3 domain is well conserved. The sequences begin to diverge at the second EGF-like domain, and the carboxyterminal end is where most of the diversity is amongst the different enzymes and slice variants. A recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Higher concentrations of antibody may be needed for samples from more distantly related species. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. FOR RESEARCH USE ONLY; NOT FOR USE IN HUMANS.
Storage
The undiluted antibody solution is stable for approximately 6 months at 4C. For longer storage, store at -20C.
